Difference between revisions of "Part:BBa K3257044"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3257044 short</partinfo>
 
<partinfo>BBa_K3257044 short</partinfo>
  
 
Cre recombinase is responsible for recombination of reverse transcribed cDNA, which is also one of the most important processes in our mutation library construction. We expressed Cre recombinase in E.coli and measured its recombination efficiency.
 
Cre recombinase is responsible for recombination of reverse transcribed cDNA, which is also one of the most important processes in our mutation library construction. We expressed Cre recombinase in E.coli and measured its recombination efficiency.
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 9: Line 9:
  
 
<!-- -->
 
<!-- -->
<span class='h3bb'>Sequence and Features</span>
+
<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K3257044 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3257044 SequenceAndFeatures</partinfo>
  

Revision as of 11:23, 9 September 2019

Cre Recombinase

Cre recombinase is responsible for recombination of reverse transcribed cDNA, which is also one of the most important processes in our mutation library construction. We expressed Cre recombinase in E.coli and measured its recombination efficiency.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]