Difference between revisions of "Part:BBa K3257031"
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This part is a mutant of a gene encoding EGFP, and it is nonsense mutated at its 158th amino acid residual. We used this mutated EGFP as one of the targets of our directed evolution process. After expression in BL21(DE3), we measured its fluorescence intensity together with wild type EGFP to show its inability of fluorescing.
 
This part is a mutant of a gene encoding EGFP, and it is nonsense mutated at its 158th amino acid residual. We used this mutated EGFP as one of the targets of our directed evolution process. After expression in BL21(DE3), we measured its fluorescence intensity together with wild type EGFP to show its inability of fluorescing.
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Revision as of 10:56, 9 September 2019


EGFP_Q158X

This part is a mutant of a gene encoding EGFP, and it is nonsense mutated at its 158th amino acid residual. We used this mutated EGFP as one of the targets of our directed evolution process. After expression in BL21(DE3), we measured its fluorescence intensity together with wild type EGFP to show its inability of fluorescing.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]