Difference between revisions of "Part:BBa K2933010"

(→‎Molecular cloning)
(→‎Molecular cloning)
Line 25: Line 25:
 
<p style="text-align: center;">
 
<p style="text-align: center;">
 
   [[File:TJUSLS China--MUS-2-PCR.png|500px]]<br>
 
   [[File:TJUSLS China--MUS-2-PCR.png|500px]]<br>
 +
'''Figure 1.'''  Left: The PCR result of MUS-2. Right: The verification results by enzyme digestion.<br>
 +
</p>
 +
After verification, it was determined that the construction is successful.

Revision as of 09:14, 8 September 2019


subclass B1 metallo-beta-lactamase MUS-2, codon optimized in E. coli

This part encodes a protein called MUS-2, which is a metallo-beta-lactamase of subclass B2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

MUS-2 is 啊

Molecular cloning

We used the vector pGEX-6p-1 to construct our expression plasmid.

TJUSLS China--MUS-2-PCR.png
Figure 1. Left: The PCR result of MUS-2. Right: The verification results by enzyme digestion.

After verification, it was determined that the construction is successful.