Difference between revisions of "Part:BBa K3257043:Design"

 
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===Design Notes===
 
===Design Notes===
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No.
 
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We mutated the 3734th nucleotide from A to T of BBa_K3257042 to lower the fidelity of MMLV reverse transcriptase.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Wen-Hui Zhang, Evguenia S. Svarovskaia, Rebekah Barr, and Vinay K. Pathak. Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine–thymine tracts[J]. Proc Natl Acad Sci U S A. 2002 Jul 23; 99(15): 10090–10095

Latest revision as of 15:04, 6 September 2019


Lower Fidelity MMLV gag-pol Polyprotein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
    Illegal BglII site found at 1286
    Illegal BglII site found at 2592
    Illegal BglII site found at 3824
    Illegal BamHI site found at 2609
    Illegal BamHI site found at 2915
    Illegal XhoI site found at 940
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1825
    Illegal AgeI site found at 3396
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated the 3734th nucleotide from A to T of BBa_K3257042 to lower the fidelity of MMLV reverse transcriptase.

Source

Mutated from MMLV genome

References

Wen-Hui Zhang, Evguenia S. Svarovskaia, Rebekah Barr, and Vinay K. Pathak. Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine–thymine tracts[J]. Proc Natl Acad Sci U S A. 2002 Jul 23; 99(15): 10090–10095