Difference between revisions of "Part:BBa K3257041"

 
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<partinfo>BBa_K3257041 short</partinfo>
 
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This part is a mutant of a gene encoding Chl, a resistance gene of chloramphenicol, and it is nonsense mutated at its 190th amino acid residual. We used this mutated Chl as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the containing chloramphenicol to show its inability of being resistant to chloramphenicol.
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This part is a mutant of a gene encoding ChlR, a resistance gene of chloramphenicol, and it is nonsense mutated at its 190th amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the containing chloramphenicol to show its inability of being resistant to chloramphenicol.
  
 
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Revision as of 14:56, 6 September 2019


Chloramphenicol Resistance Gene Q190X (ChlR_Q190X)

This part is a mutant of a gene encoding ChlR, a resistance gene of chloramphenicol, and it is nonsense mutated at its 190th amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the containing chloramphenicol to show its inability of being resistant to chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]