Difference between revisions of "Part:BBa K3257036"

 
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<partinfo>BBa_K3257036 short</partinfo>
 
<partinfo>BBa_K3257036 short</partinfo>
  
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This part is a mutant of a gene encoding Chl, a resistance protein of chloramphenicol, and it is nonsense mutated at its 92nd amino acid residual. We used this mutated Chl as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the number of colonies grown on a medium plate containing chloramphenicol to show its inability of being resistant to chloramphenicol.
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This part is a mutant of a gene encoding ChlR, a resistance protein of chloramphenicol, and it is nonsense mutated at its 92nd amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the number of colonies grown on a medium plate containing chloramphenicol to show its inability of being resistant to chloramphenicol.
  
 
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Revision as of 13:03, 6 September 2019


Chloramphenicol Resistance Gene Y92X (ChlR_Y92X)

This part is a mutant of a gene encoding ChlR, a resistance protein of chloramphenicol, and it is nonsense mutated at its 92nd amino acid residual. We used this mutated ChlR as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the number of colonies grown on a medium plate containing chloramphenicol to show its inability of being resistant to chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]