Difference between revisions of "Part:BBa K3257036"

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Chloramphenicol Resistance Gene Y92X (ChlR_Y92X)

This part is a mutant of a gene encoding Chl, a resistance protein of chloramphenicol, and it is nonsense mutated at its 92nd amino acid residual. We used this mutated Chl as one of the targets of our directed evolution process. After expression in BL21(DE3), we counted the number of colonies grown on a medium plate containing chloramphenicol to show its inability of being resistant to chloramphenicol.

Sequence and Features