Difference between revisions of "Part:BBa K3198001"

 
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HicB functions as an mRNA interferase antitoxin. Expression of HicB (145 aa) prevented HicA-mediated inhibition of cell growth. HicB neutralizes HicA and therefore functions as an antitoxin. HicB could resuscitate cells inhibited by HicA.
 
HicB functions as an mRNA interferase antitoxin. Expression of HicB (145 aa) prevented HicA-mediated inhibition of cell growth. HicB neutralizes HicA and therefore functions as an antitoxin. HicB could resuscitate cells inhibited by HicA.
  
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===Usage and Biology===
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<ul>
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<li>Stress externally introduced via a synthetic construct can be quantified via this part. Levels of fluorescence will be dependent on the amount of stress experienced by the cell since transcription will be driven by the stress-induced promoter. </li>
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<li>Basal stress levels (inherent stress levels, in addition to the stress brought about by the PhtpG1-mRFP construct itself) can be measured. </li>
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<li>Characterization was done in DH5α and BL21 Star (DE3). Ceroni et al. (2018) have also successfully tested the PhtpG1 promoter in MG1655 and DH10B. </li>
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<li><u><b>To note</b></u>: Plasmid backbone part that is inserted <b> should not </b> have the same antibiotic resistance as the bacteria it is transformed into.</li>
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</ul><br>
  
  

Revision as of 09:03, 31 August 2019


HicB

This part contains the antitoxin component of a type II toxin-antitoxin (TA) system. It functions as an mRNA interferase antitoxin; overexpression prevents HicA-mediated cessation of cell growth and cell death. HicB functions as an mRNA interferase antitoxin. Expression of HicB (145 aa) prevented HicA-mediated inhibition of cell growth. HicB neutralizes HicA and therefore functions as an antitoxin. HicB could resuscitate cells inhibited by HicA.

Usage and Biology

  • Stress externally introduced via a synthetic construct can be quantified via this part. Levels of fluorescence will be dependent on the amount of stress experienced by the cell since transcription will be driven by the stress-induced promoter.
  • Basal stress levels (inherent stress levels, in addition to the stress brought about by the PhtpG1-mRFP construct itself) can be measured.
  • Characterization was done in DH5α and BL21 Star (DE3). Ceroni et al. (2018) have also successfully tested the PhtpG1 promoter in MG1655 and DH10B.
  • To note: Plasmid backbone part that is inserted should not have the same antibiotic resistance as the bacteria it is transformed into.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 48
    Illegal BsaI.rc site found at 88