Difference between revisions of "Part:BBa K3039010:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K3039010 short</partinfo>
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<partinfo>BBa_K3039010 SequenceAndFeatures</partinfo>
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===Design Notes===
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The entire amino acid sequence was codon optimised for <I>E. coli</I> by IDT’s on-line Codon Optimisation tool ensuring that there were no forbidden restriction sites, BsaI or SapI, to allow for TypeIIS assembly.
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===Source===
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Wild type amino acid sequence originates from <I>Ideonella sakaiensis.</I> Mutations have been directed by reference to the literature. Resulting DNA sequences were codon optimized by the IDT online software.
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===References===

Latest revision as of 10:28, 30 August 2019


SP_lamB-PETase T88A_S93M_S121E_W159F_D186H_R280A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 654
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The entire amino acid sequence was codon optimised for E. coli by IDT’s on-line Codon Optimisation tool ensuring that there were no forbidden restriction sites, BsaI or SapI, to allow for TypeIIS assembly.

Source

Wild type amino acid sequence originates from Ideonella sakaiensis. Mutations have been directed by reference to the literature. Resulting DNA sequences were codon optimized by the IDT online software.

References