Difference between revisions of "Part:BBa K2909000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We designed the HiBiT tag to be standardized to the C. reinhardtii MoClo Kit (Crozet et al. 2018). | + | We designed the HiBiT tag to be standardized to the C. reinhardtii MoClo Kit (Crozet et al. 2018).<br> |
BbsI sites and fusion sites corresponding to the B2 position (N-terminal tag) were added on both side to be compatible with the MoClo assembly standard. | BbsI sites and fusion sites corresponding to the B2 position (N-terminal tag) were added on both side to be compatible with the MoClo assembly standard. | ||
Revision as of 08:00, 29 August 2019
HiBiT-B2 MoClo C. reinhardtii
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed the HiBiT tag to be standardized to the C. reinhardtii MoClo Kit (Crozet et al. 2018).
BbsI sites and fusion sites corresponding to the B2 position (N-terminal tag) were added on both side to be compatible with the MoClo assembly standard.
Source
This part comes from Promega (Schwinn et al. 2018).
References
- Schwinn, M. K. et al. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chem. Biol. 13, 467–474 (2018).
- Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
- 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).