Difference between revisions of "Part:BBa K2909001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We designed the HiBiT tag to be standardized to the C. reinhardtii MoClo Kit (Crozet et al. 2018). | + | We designed the HiBiT tag to be standardized to the C. reinhardtii MoClo Kit (Crozet et al. 2018).<br> |
| − | BbsI sites and fusion sites corresponding to the B5 position (C-terminal tag)were added on both side to be compatible with the MoClo assembly standard. | + | BbsI sites and fusion sites corresponding to the B5 position (C-terminal tag) were added on both side to be compatible with the MoClo assembly standard.<br> |
A stop codon (TAA) was added at the 3' end of the HiBiT tag as it is a C-terminal tag. | A stop codon (TAA) was added at the 3' end of the HiBiT tag as it is a C-terminal tag. | ||
Revision as of 07:56, 29 August 2019
HiBiT-B5 MoClo C. reinhardtii
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed the HiBiT tag to be standardized to the C. reinhardtii MoClo Kit (Crozet et al. 2018).
BbsI sites and fusion sites corresponding to the B5 position (C-terminal tag) were added on both side to be compatible with the MoClo assembly standard.
A stop codon (TAA) was added at the 3' end of the HiBiT tag as it is a C-terminal tag.
Source
This part comes from Promega (Schwinn et al. 2018).
References
- Schwinn, M. K. et al. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chem. Biol. 13, 467–474 (2018).
- Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
- 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).