Difference between revisions of "Part:BBa K2909008:Design"

 
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===Design Notes===
 
===Design Notes===
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===Source===
 
===Source===
  
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The LPAAT-A enzyme (BBa_K2909002) comes from the predicted sequence from the E. guineensis genome sequencing.<br>
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NCBI Reference Sequence: XM_010933834.1<br>
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https://www.ncbi.nlm.nih.gov/nuccore/XM_010933834.1<br><br>
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The N-terminal HiBiT tag (BBa_K2909000) comes from Promega (Schwinn et al. 2018).<br><br>
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All the other parts comes from the Chlamydomonas reinhardtii MoClo Kit (Crozet et al. 2018). <br>
  
 
===References===
 
===References===
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<ol>
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<li> Schwinn, M. K. et al. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chem. Biol. 13, 467–474 (2018). </li>
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<li> Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018). </li>
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<li> 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).</li>
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</ol>

Latest revision as of 15:20, 28 August 2019


ParoR_CloverGFP-HiBiT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 1567
    Illegal PstI site found at 2806
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 1567
    Illegal NheI site found at 269
    Illegal PstI site found at 2806
    Illegal NotI site found at 1779
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 1567
    Illegal BamHI site found at 11
    Illegal XhoI site found at 2320
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 1567
    Illegal PstI site found at 2806
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5
    Illegal EcoRI site found at 1567
    Illegal PstI site found at 2806
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3466


Design Notes

Source

The LPAAT-A enzyme (BBa_K2909002) comes from the predicted sequence from the E. guineensis genome sequencing.
NCBI Reference Sequence: XM_010933834.1
https://www.ncbi.nlm.nih.gov/nuccore/XM_010933834.1

The N-terminal HiBiT tag (BBa_K2909000) comes from Promega (Schwinn et al. 2018).

All the other parts comes from the Chlamydomonas reinhardtii MoClo Kit (Crozet et al. 2018).

References

  1. Schwinn, M. K. et al. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chem. Biol. 13, 467–474 (2018).
  2. Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
  3. 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).