Difference between revisions of "Part:BBa K2909000:Design"
Line 1: Line 1:
 +
 +
__NOTOC__
 +
<partinfo>BBa_K2909000 short</partinfo>
 +
 +
<partinfo>BBa_K2909000 SequenceAndFeatures</partinfo>
  
  
Line 5: Line 10:
 
We designed the HiBiT tag to be standardized to the C. reinhardtii MoClo Kit (Crozet et al. 2018).
 
We designed the HiBiT tag to be standardized to the C. reinhardtii MoClo Kit (Crozet et al. 2018).
 
BbsI sites and fusion sites corresponding to the B2 position (N-terminal tag) were added on both side to be compatible with the MoClo assembly standard.
 
BbsI sites and fusion sites corresponding to the B2 position (N-terminal tag) were added on both side to be compatible with the MoClo assembly standard.
−
 
−
 
  
 
===Source===
 
===Source===

Revision as of 14:44, 26 August 2019


HiBiT-B2 MoClo C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed the HiBiT tag to be standardized to the C. reinhardtii MoClo Kit (Crozet et al. 2018). BbsI sites and fusion sites corresponding to the B2 position (N-terminal tag) were added on both side to be compatible with the MoClo assembly standard.

Source

This part comes from Promega (Schwinn et al. 2018).

References

Schwinn, M. K. et al. CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide. ACS Chem. Biol. 13, 467–474 (2018). Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).