Difference between revisions of "Part:BBa K2973007:Design"
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===Design Notes===
 
===Design Notes===
 
The toehold switch sequence was taken from Pardee et al., 2016 .
 
The toehold switch sequence was taken from Pardee et al., 2016 .
−
We deleted the signal peptide from the beta-lactamase sequence in order to do our in vitro experiments.
+
Beta-lactamase needs to be truncated in its N-terminal end in order to work properly. Therefore, we deleted the signal peptide from its sequence in order to do our in vitro experiments
  
  

Revision as of 18:10, 17 August 2019


32B Toehold Switch_β-lactamase_no signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The toehold switch sequence was taken from Pardee et al., 2016 . Beta-lactamase needs to be truncated in its N-terminal end in order to work properly. Therefore, we deleted the signal peptide from its sequence in order to do our in vitro experiments


Source

Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059.

References

Pardee, Keith, et al. “Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components.” Cell, vol. 165, no. 5, 2016, pp. 1255–1266., doi:10.1016/j.cell.2016.04.059.

Green, Alexander A., et al. “Toehold Switches: De-Novo-Designed Regulators of Gene Expression.” Cell, vol. 159, no. 4, 2014, pp. 925–939., doi:10.1016/j.cell.2014.10.002.

Qureshi, Sohail A. “β-Lactamase: an Ideal Reporter System for Monitoring Gene Expression in Live Eukaryotic Cells.” BioTechniques, vol. 42, no. 1, 2007, pp. 91–96., doi:10.2144/000112292.

Boehle, Katherine E., et al. “Paper-Based Enzyme Competition Assay for Detecting Falsified β-Lactam Antibiotics.” ACS Sensors, vol. 3, no. 7, 2018, pp. 1299–1307., doi:10.1021/acssensors.8b00163.