Difference between revisions of "Part:BBa K3174003"

 
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This part has the blue fluorescent protein (mTagBFP) gene inserted behind a strong promoter and medium ribosome binding site.
 
This part has the blue fluorescent protein (mTagBFP) gene inserted behind a strong promoter and medium ribosome binding site.
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UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other biobricks that had been similarly transformed into the burden strain.
 
  
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===Usage and Biology===
 
===Usage and Biology===
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UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other biobricks that had been similarly transformed into the burden strain.
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Revision as of 20:01, 8 August 2019


Blue Fluorescent Protein with Strong Promoter and Medium RBS

This part has the blue fluorescent protein (mTagBFP) gene inserted behind a strong promoter and medium ribosome binding site.


Usage and Biology

UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other biobricks that had been similarly transformed into the burden strain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]