Difference between revisions of "Part:BBa K3128004:Design"

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===Design Notes===
 
===Design Notes===
 
We use the BBA J04450 and remove the RFP gene to put the NanoLuc gene but this part does not contain any restriction site to remove only the RFP. We add 2 restrictions sites on the biobrick to allow us to remove it and put the NanoLuc. BglII in 3' and BamHI in 5'. Those sites are still in the biobrick.
 
We use the BBA J04450 and remove the RFP gene to put the NanoLuc gene but this part does not contain any restriction site to remove only the RFP. We add 2 restrictions sites on the biobrick to allow us to remove it and put the NanoLuc. BglII in 3' and BamHI in 5'. Those sites are still in the biobrick.
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===Source===
 
===Source===

Revision as of 08:25, 6 August 2019


NanoLuciferase reporter for BACTH assay


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use the BBA J04450 and remove the RFP gene to put the NanoLuc gene but this part does not contain any restriction site to remove only the RFP. We add 2 restrictions sites on the biobrick to allow us to remove it and put the NanoLuc. BglII in 3' and BamHI in 5'. Those sites are still in the biobrick.

Source

PLac-RBS and the termintors are from iGEM plates from Bba_J04450 The NanoLuc gene is from Promega vector's

References