Difference between revisions of "Part:BBa K3128004:Design"
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===Source=== | ===Source=== | ||
− | PLac-RBS and the termintors are from iGEM plates | + | PLac-RBS and the termintors are from iGEM plates from Bba_J04450 |
− | The NanoLuc gene is from Promega | + | The NanoLuc gene is from Promega vector's |
===References=== | ===References=== |
Revision as of 08:25, 6 August 2019
NanoLuciferase reporter for BACTH assay
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We use the BBA J04450 and remove the RFP gene to put the NanoLuc gene but this part does not contain any restriction site to remove only the RFP. We add 2 restrictions sites on the biobrick to allow us to remove it and put the NanoLuc. BglII in 3' and BamHI in 5'. Those sites are still in the biobrick.
Source
PLac-RBS and the termintors are from iGEM plates from Bba_J04450 The NanoLuc gene is from Promega vector's