Difference between revisions of "Part:BBa K2916003:Design"
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===Design Notes=== | ===Design Notes=== | ||
The sequence was extracted from the pET16b-His-ArgRS plasmid and expressed in the BL21 (DE3) competent E.coli strain | The sequence was extracted from the pET16b-His-ArgRS plasmid and expressed in the BL21 (DE3) competent E.coli strain | ||
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===Source=== | ===Source=== | ||
Revision as of 15:55, 23 July 2019
GlnRS protein equipped with a 6x HIS affinity tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 54
Illegal BglII site found at 1002
Illegal XhoI site found at 1663 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1608
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was extracted from the pET16b-His-ArgRS plasmid and expressed in the BL21 (DE3) competent E.coli strain
Source
http://www.addgene.org/124104/
References
Cell-free translation reconstituted with purified components. Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T. Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802. 10.1038/90802 PubMed 11479568