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| | ===Source=== | | ===Source=== |
| | | | |
| − | atgagaggatcgcatcaccatcaccatcacggatccgcatgcgatgacgatgacaaaagcaacagcaccgctgagatccgtcaggcgtttctcgactttttccatagtaagggacatcaggtagttgccagcagctccctggtaccccataacgacccaactttgttgtttaccaacgccgggatgaaccagttcaaggatgtgttccttgggctcgacaagcgtaattattcccgcgctaccacttcccaacgctgcgtgcgtgcgggtggtaaacacaacgacctggaaaacgtcggttacaccgcgcgtcaccataccttcttcgaaatgctgggcaacttcagcttcggcgactatttcaaacacgatgccattcagtttgcatgggaactgctgaccagcgaaaaatggtttgccctgccgaaagagcgtctgtgggttaccgtctatgaaagcgacgacgaagcctacgaaatctgggaaaaagaagtagggatcccgcgcgaacgtattattcgcatcggcgataacaaaggtgcgccatacgcatctgacaacttctggcagatgggtgacactggtccgtgcggcccgtgcaccgaaatcttctacgatcacggcgaccacatttgggggggccctccgggaagcccggaagaagacggcgaccgctacattgagatctggaacatcgtcttcatgcagttcaaccgccaggccgatggcacgatggaaccgctgccgaagccgtctgtagataccggtatgggtctggagcgtattgctgcggtgctgcaacacgttaactctaactatgacatcgacctgttccgcacgctgatccaggcggtagcgaaagtcactggcgcaaccgatctgagcaataaatcgctgcgcgtaatcgctgaccacattcgttcttgtgcgttcctaatcgcggatggcgtaatgccgtccaatgaaaaccgtggttatgtactgcgtcgtatcattcgtcgcgcagtgcgtcacggtaatatgctcggcgcgaaagaaaccttcttctacaaactggttggtccgctgatcgacgttatgggctctgcgggtgaagacctgaaacgccagcaggcgcaggttgagcaggtgctgaagactgaagaagagcagtttgctcgtactctggagcgcggtctggcgttgctggatgaagagctggcaaaactttctggtgatacgctggatggtgaaactgctttccgtctgtacgacacctatggcttcccggttgacctgacggctgatgtttgtcgtgagcgcaacatcaaagttgacgaagctggttttgaagctgcaatggaagagcagcgtcgtcgcgcgcgcgaagccagcggctttggtgccgattacaacgcaatgatccgtgttgacagtgcatctgaatttaaaggctatgaccatctggaactgaacggcaaagtgactgcgctgtttgttgatggtaaagcggttgatgccatcaatgcaggccaggaagctgtggtcgtgctggatcaaacgccattctatgcggaatccggcggtcaggttggcgataaaggcgaactgaaaggcgctaacttctcctttgcggtggaagatacgcagaaatacggccaggcgattggtcacatcggtaaacttgctgcgggttctctgaaagtgggcgacgcggtgcaggctgatgttgatgaggctcgtcgcgcccgtattcgtctgaatcactccgcaacgcacctgatgcacgctgcgctgcgccaggttctgggtactcatgtatcgcagaaaggttcactggttaacgacaaggtgctgcgcttcgacttctcacacaacgaagcgatgaaaccagaagagattcgtgcggtcgaagacctggtgaacacacagattcgtcgcaatttgccgatcgaaaccaacatcatggatctcgaagcggcgaaagcgaaaggtgcgatggcgctgttcggcgagaagtatgatgagcgcgtacgcgtgctgagcatgggcgatttctctaccgagttgtgtggcggtactcacgccagccgcactggtgatattggtctgttccgcatcatctctgaatcgggtactgctgcaggcgttcgtcgtatcgaagcggtaaccggagaaggtgctatcgccaccgttcatgcagacagcgatcgcttaagcgaagtcgcgcatctgctgaaaggcgatagcaataatctggctgataaagtgcgctcagtactggaacgtacgcgtcagctggaaaaagagttacaacagcttaaagaacaagctgccgcacaggagagcgcaaatctttccagtaaggcaattgatgttaatggtgttaagctgttggttagcgagcttagcggtgttgagccgaaaatgttgcgtaccatggttgacgatttaaaaaatcagctggggtcgacaattatcgtgctggcaacggtagtcgaaggtaaggtttctctgattgcaggcgtatctaaggacgtcacagatcgtgtgaaagcaggggaactgattggtatggtcgctcagcaggtgggcggcaagggtggtggacgtcctgacatggcgcaagccggtggtacggatgctgcggccttacctgcagcgttagccagtgtgaaaggctgggtcagcgcgaaattgcaataa
| + | Escherichia coli gene alaS |
| | | | |
| | ===References=== | | ===References=== |
Revision as of 14:49, 22 July 2019
ArgRS protein equipped with a 10x HIS affinity tag
Assembly Compatibility:
- 10
- 12
- 21
- 23
- 25
- 1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 76
Illegal SapI.rc site found at 1495
Design Notes
The CAA with be mutated into a TAA stop codon
Source
Escherichia coli gene alaS
References
