Difference between revisions of "Part:BBa J119443"

Latest revision as of 21:28, 18 July 2019

RET Promoter G-quadruplex and i-Motif

The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The G-quadruplex is shown on the coding strand of this sequence. If the part was ligated into a plasmid that had been digested with Eco RI, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand). This i-motif/G-quadruplex-forming sequence comes from the human RET promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_J119443 short</partinfo>
-The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The i-motif is shown on the coding strand in this sequence. The i-motif/G-quadruplex-forming sequence comes from the human RET promoter. The G-quadruplex is shown on the coding strand of this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, however, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand).
+The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The G-quadruplex is shown on the coding strand of this sequence. If the part was ligated into a plasmid that had been digested with Eco RI, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand).  This i-motif/G-quadruplex-forming sequence comes from the human RET promoter.
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