Difference between revisions of "Part:BBa J119439"
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<partinfo>BBa_J119439 short</partinfo>
 
<partinfo>BBa_J119439 short</partinfo>
  
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The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The G-quadruplex is shown on the coding strand in this sequence.  This places the i-motif sequence on the template strand. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand).  The i-motif/G-quadruplex-forming sequence comes from the G- and C-rich repeats in human telomeres.
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The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The G-quadruplex is shown on the coding strand in this sequence.  This places the i-motif sequence on the template strand. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand).  The i-motif/G-quadruplex-forming sequence comes from the human BCL-2 promoter
  
  

Revision as of 21:21, 18 July 2019


BCL-2 Promoter G-quadruplex and i-Motif

The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The G-quadruplex is shown on the coding strand in this sequence. This places the i-motif sequence on the template strand. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand). The i-motif/G-quadruplex-forming sequence comes from the human BCL-2 promoter


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]