Difference between revisions of "Part:BBa J119438"
 
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<partinfo>BBa_J119438 short</partinfo>
 
<partinfo>BBa_J119438 short</partinfo>
  
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The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand.  The i-motif is shown on the coding strand in this sequence.  
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The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The i-motif is shown on the coding strand of this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand).  The i-motif/G-quadruplex-forming sequence comes from the human BCL-2 promoter.
  
 
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Latest revision as of 21:18, 18 July 2019


BCL-2 promoter i-Motif and G quadruplex

The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. The i-motif is shown on the coding strand of this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand). The i-motif/G-quadruplex-forming sequence comes from the human BCL-2 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]