Difference between revisions of "Part:BBa J119443"
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<partinfo>BBa_J119443 short</partinfo> | <partinfo>BBa_J119443 short</partinfo> | ||
| − | The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The | + | The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The G-quadruplex is shown on the coding strand in this sequence. The i-motif/G-quadruplex-forming sequence comes from the human RET promoter. The G-quadruplex is shown on the coding strand of this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, however, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand). |
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Revision as of 20:56, 18 July 2019
RET Promoter G-quadruplex and i-Motif
The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The G-quadruplex is shown on the coding strand in this sequence. The i-motif/G-quadruplex-forming sequence comes from the human RET promoter. The G-quadruplex is shown on the coding strand of this sequence. If the part was to be ligated into a plasmid that had been digested with Eco RI, however, it could be ligated in either orientation (i-motif on the coding strand or G-quadruplex on the coding strand).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]