Difference between revisions of "Part:BBa J119440"
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<partinfo>BBa_J119440 short</partinfo> | <partinfo>BBa_J119440 short</partinfo> | ||
| − | The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The | + | The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The G-quadruplex is shown on the coding strand in this sequence. The i-motif/G-quadruplex-forming sequence comes from the human c-Myc promoter's Nuclease Hypersenstive Element III. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either oreintation (i-motif on the coding strand or G-quadruplex on the coding strand). |
| − | + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 20:50, 18 July 2019
c-Myc Promoter G-quadruplex and i-Motif
The part forms an i-motif and/or G quadruplex, and has sticky ends on both 5' ends. These ends allow the part to be inserted into an Eco RI cut site with either strand on the template or coding strand. The G-quadruplex is shown on the coding strand in this sequence. The i-motif/G-quadruplex-forming sequence comes from the human c-Myc promoter's Nuclease Hypersenstive Element III. If the part was to be ligated into a plasmid that had been digested with Eco RI, it could be ligated in either oreintation (i-motif on the coding strand or G-quadruplex on the coding strand). Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]