Difference between revisions of "Part:BBa K2967002:Design"
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===References=== | ===References=== | ||
| + | Wang,B., Kitney,R.I., Joly,N. and Buck,M. (2011) Engineering | ||
| + | modular and orthogonal genetic logic gates for robust digital-like | ||
| + | synthetic biology. Nat. Commun., 2, 508. | ||
Latest revision as of 15:59, 17 July 2019
a 54 -dependent hrpL promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of the protein complex HrpRS specifically acting on the hrpL promoter region has not been investigated yet, so the hrpL promoter region in the amplifier is relatively long. In order to ensure that the transcription initiation is completed normally, we do not change the sequence.
Source
it is from the hrp (hypersensitive response and pathogenicity) gene regulatory network for Type III secretion in Pseudomonas syringae.
References
Wang,B., Kitney,R.I., Joly,N. and Buck,M. (2011) Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology. Nat. Commun., 2, 508.