Difference between revisions of "Part:BBa K3182000"

Revision as of 17:13, 11 July 2019

pT7-CBDcipA-AsPink

A cellulose binding domain (CBD) from Clostridium thermocellum which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added at the end, clevage with thrombin will add one glycine and one serine to the C-terminal of the fusion protein. The part has a AsPink fused to the CBD to ensure easy trackable expression and characterization of the CBD. The part has a very strong expression with a T7 promotor as well as a 5'UTR (BBa_K1758100) region which has been shown to further increase expression in E. coli .

Figure 1. E. coli BL21 cells expressing this biobrick, incubated for 16 hours at 16 degrees Celsius.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 580
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3182000 short</partinfo>
-A cellulose binding domain (CBD) which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added at the end, clevage with thrombin will add one glycine and one serine to the start of the C-terminal of the fusion tail. The part has a AsPink fused to the CBD to ensure easy trackable expression and characterization of the CBD. The part has a very strong expression with a T7 promotor as well as a 5'UTR region which has been shown to further increase expression in E. coli.
+A cellulose binding domain (CBD) from Clostridium thermocellum which can be used to purify or attach proteins to cellulose. The part also has a flexible GS-linker with a thrombin site added at the end, clevage with thrombin will add one glycine and one serine to the C-terminal of the fusion protein. The part has a AsPink fused to the CBD to ensure easy trackable expression and characterization of the CBD. The part has a very strong expression with a T7 promotor as well as a 5'UTR (<partinfo>BBa_K1758100</partinfo>) region which has been shown to further increase expression in E. coli .
 [[File:T--Linkoping_Sweden--CBD-AsPink.jpeg|430px|thumb|center|<b>Figure 1.</b> E. coli BL21 cells expressing this biobrick, incubated for 16 hours at 16 degrees Celsius.]]