Difference between revisions of "Part:BBa K2905001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Codon optimized | + | Codon optimized; 3A assembly scar sites included between promoter, RBS, and coding region. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | IDT synthesized DNA | + | IDT synthesized DNA. |
===References=== | ===References=== | ||
Latest revision as of 18:36, 24 June 2019
Constitutively expressed 6-His-TEV-MttB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 104 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1220
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 869
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1046
Design Notes
Codon optimized; 3A assembly scar sites included between promoter, RBS, and coding region.
Source
IDT synthesized DNA.