Difference between revisions of "Part:BBa J119412"
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− | This part includes the -35 and -10 elements of the strong pTac promoter. The human c-Myc promoter was found to have a sequence capable of forming an i-motif in its nuclease hypersensitive element III. This i-motif forming sequence was modified to fit around the -35 region of the pTac promoter. | + | This part includes the -35 and -10 elements of the strong pTac promoter. The human c-Myc promoter was found to have a sequence capable of forming an i-motif in its nuclease hypersensitive element III. This i-motif forming sequence was modified to fit on the template strand, around the -35 region of the pTac promoter. |
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Latest revision as of 16:13, 5 January 2019
IM1 - i-motif sequence on pTac template strand
This part includes the -35 and -10 elements of the strong pTac promoter. The human c-Myc promoter was found to have a sequence capable of forming an i-motif in its nuclease hypersensitive element III. This i-motif forming sequence was modified to fit on the template strand, around the -35 region of the pTac promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]