Difference between revisions of "Part:BBa J119412"

 
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<partinfo>BBa_J119412 short</partinfo>
 
<partinfo>BBa_J119412 short</partinfo>
  
This part includes the -35 and -10 elements of the strong pTac promoter.  The human c-Myc promoter was found to have a sequence capable of forming an i-motif in its nuclease hypersensitive element III.  This i-motif forming sequence was modified to fit around the -35 region of the pTac promoter.   
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This part includes the -35 and -10 elements of the strong pTac promoter.  The human c-Myc promoter was found to have a sequence capable of forming an i-motif in its nuclease hypersensitive element III.  This i-motif forming sequence was modified to fit on the template strand, around the -35 region of the pTac promoter.   
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 16:13, 5 January 2019


IM1 - i-motif sequence on pTac template strand

This part includes the -35 and -10 elements of the strong pTac promoter. The human c-Myc promoter was found to have a sequence capable of forming an i-motif in its nuclease hypersensitive element III. This i-motif forming sequence was modified to fit on the template strand, around the -35 region of the pTac promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]