Difference between revisions of "Part:BBa J119413"

Latest revision as of 16:12, 5 January 2019

G41 - i-motif sequence on pTac coding strand

This promoter is pTac with an i-motif forming sequence on the coding strand. The i-motif sequence was a modified form of the human c-Myc promoter's Nuclease Hypersensitive Element III. The -10 element is included as a loop in the i-motif. The p-Tac -35 and -10 elements were adjusted to have 17 base pairs between them, the optimal spacing, and an i-motif sequence was chosen to fit around the -10 element and in the center of the promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_J119413 short</partinfo>
-This sequence is the reverse of BBa_J119412.  It has an i-motif forming sequence on the top strand, contained within a modified pTac promoter.  The -10 element is included as a loop in the i-motif.  The p-Tac -35 and -10 elements were adjusted to have 17 base pairs between them, the optimal spacing, and an i-motif sequence was chosen to fit around the -10 element and in the center of the promoter.
+This promoter is pTac with an i-motif forming sequence on the coding strand.  The i-motif sequence was a modified form of the human c-Myc promoter's Nuclease Hypersensitive Element III.  The -10 element is included as a loop in the i-motif.  The p-Tac -35 and -10 elements were adjusted to have 17 base pairs between them, the optimal spacing, and an i-motif sequence was chosen to fit around the -10 element and in the center of the promoter.
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