Difference between revisions of "Part:BBa K2448001:Design"
 
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===Design Notes===
 
===Design Notes===
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This protein has been specifically optimized for Escherichia coli K12 with help of JCAT ([http://www.jcat.de/]). Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.
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This sequence has been specifically optimised for ''Escherichia coli'' K12 with the help of [http://www.jcat.de/ JCAT]. Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.
  
 
===Source===
 
===Source===
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===References===
 
===References===
  
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[1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the Aequorea victoria green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30.
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[1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the ''Aequorea victoria'' green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30.
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[2] Addgene plasmid # 53976
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Latest revision as of 18:53, 31 December 2018


mEmerald


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been specifically optimised for Escherichia coli K12 with the help of [http://www.jcat.de/ JCAT]. Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.

Source

DNA synthesis

References

[1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the Aequorea victoria green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30.