Difference between revisions of "Part:BBa K2448054"
 
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This part is an ''E. coli'' codon optimized version of the D-Psicose 3-epimerase (Dpe) from ''Clostridium cellulolyticum'' str. ATCC 35319
 
This part is an ''E. coli'' codon optimized version of the D-Psicose 3-epimerase (Dpe) from ''Clostridium cellulolyticum'' str. ATCC 35319
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(Uniprot B8I944) with a C-terminal HisTag [[Part:BBa_K2448053|BBa_K2448053]]) equipped with a strong RBS ([[Part:BBa_K2448019|BBa_K2448019]]) and placed under the control of the pTacI promoter ([[Part:BBa_K864400|BBa_K864400]]).
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(Uniprot B8I944) with a C-terminal HisTag ([[Part:BBa_K2448053|BBa_K2448053]]) equipped with a strong RBS ([[Part:BBa_K2448019|BBa_K2448019]]) and placed under the control of the pTacI promoter ([[Part:BBa_K864400|BBa_K864400]]).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:30, 27 December 2018


D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum with a C-terminal HisTag under the contr

This part is an E. coli codon optimized version of the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum str. ATCC 35319 (Uniprot B8I944) with a C-terminal HisTag (BBa_K2448053) equipped with a strong RBS (BBa_K2448019) and placed under the control of the pTacI promoter (BBa_K864400).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    COMPATIBLE WITH RFC[1000]