Difference between revisions of "Part:BBa M50470:Design"
| Line 20: | Line 20: | ||
https://parts.igem.org/Part:BBa_K1033929 | https://parts.igem.org/Part:BBa_K1033929 | ||
https://parts.igem.org/Part:BBa_K823034 | https://parts.igem.org/Part:BBa_K823034 | ||
| + | Role of Escherichia coli cspA promoter sequences and adaptation of translational apparatus in the cold shock response: https://www.ncbi.nlm.nih.gov/pubmed/9393453 | ||
| + | Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology: https://www.ncbi.nlm.nih.gov/pubmed/29760772 | ||
| + | Agashe, V. R., & Hartl, F. (2000). Roles of molecular chaperones in cytoplasmic protein folding. Seminars in Cell & Developmental Biology, 11(1), 15-25. | ||
Revision as of 13:49, 12 December 2018
Cold Shock Sensor w/ aeBlue+3X-FLAG tag Pigment Reporter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added an aeBlue chromoprotein sequence with an RBS taken from BBa_K1033929 downstream of our cspA promoter (Bba_K328000) such that the cspA promoter controls transcription of the aeBlue chromoprotein. Behind the chromoprotein sequence is a 3X-FLAG epitope tag (Bba_K823034) that we can use for Western blotting and other protein separation experiments.
Source
aeBlue chromoprotein: BBa_K1033929 cspA promoter: Bba_K328000 3X-FLAG tag: Bba_K823034
References
https://parts.igem.org/Part:BBa_K328000 https://parts.igem.org/Part:BBa_K1033929 https://parts.igem.org/Part:BBa_K823034 Role of Escherichia coli cspA promoter sequences and adaptation of translational apparatus in the cold shock response: https://www.ncbi.nlm.nih.gov/pubmed/9393453 Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology: https://www.ncbi.nlm.nih.gov/pubmed/29760772 Agashe, V. R., & Hartl, F. (2000). Roles of molecular chaperones in cytoplasmic protein folding. Seminars in Cell & Developmental Biology, 11(1), 15-25.