Difference between revisions of "Part:BBa M50470:Design"

 
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===Design Notes===
 
===Design Notes===
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to be entered
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We added an aeBlue chromoprotein sequence with an RBS taken from Bba_K328000 downstream of our cspA promoter (Bba_K1895002) such that the cspA promoter controls transcription of the aeBlue chromoprotein. Behind the chromoprotein sequence is a 3X-FLAG epitope tag (Bba_K823034) that we can use for Western blotting and other protein separation experiments.
  
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===Source===
  
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Bba_K328000
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Bba_K1895002
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Bba_K823034
  
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===Source===
 
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all iGEM parts
 
  
 
===References===
 
===References===

Revision as of 13:38, 12 December 2018


Cold Shock Sensor w/ aeBlue+3X-FLAG tag Pigment Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added an aeBlue chromoprotein sequence with an RBS taken from Bba_K328000 downstream of our cspA promoter (Bba_K1895002) such that the cspA promoter controls transcription of the aeBlue chromoprotein. Behind the chromoprotein sequence is a 3X-FLAG epitope tag (Bba_K823034) that we can use for Western blotting and other protein separation experiments.

Source

Bba_K328000 Bba_K1895002 Bba_K823034


References