Difference between revisions of "Part:BBa K2624000:Experience"

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===Applications of BBa_K2624000===
 
===Applications of BBa_K2624000===
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To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency,although the efficiency is lower than CMV promoter.
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To improve the selectivity of our system, we utilized hTERT, the cancer-specific promoter, along with HULC, a HCC(Hepatocellular carcinoma)-specific promoter, to open an "AND" gate system to target HCC. We used pGL3-Basic vector, a promoter-less vector for the luciferase assay to determine the transcriptional activity of these promoters. We added the promoters on pGL3-Basic vector and measure the OD value of the luciferase activity. We chose SV40, a highly activated promoter in both cancer cells and normal cells as positive control (PGL3-CON). Results show that hTERT promoter has a good efficiency in cancer cells.
  
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"https://static.igem.org/mediawiki/2018/5/56/T--CPU_CHINA--hp-qidongzi.png"
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https://static.igem.org/mediawiki/2018/archive/5/56/20181017205901%21T--CPU_CHINA--hp-qidongzi.png
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Figure. The activity of the promoters
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 03:33, 8 December 2018


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Please enter how you used this part and how it worked out.

Applications of BBa_K2624000

To improve the selectivity of our system, we utilized hTERT, the cancer-specific promoter, along with HULC, a HCC(Hepatocellular carcinoma)-specific promoter, to open an "AND" gate system to target HCC. We used pGL3-Basic vector, a promoter-less vector for the luciferase assay to determine the transcriptional activity of these promoters. We added the promoters on pGL3-Basic vector and measure the OD value of the luciferase activity. We chose SV40, a highly activated promoter in both cancer cells and normal cells as positive control (PGL3-CON). Results show that hTERT promoter has a good efficiency in cancer cells.

20181017205901%21T--CPU_CHINA--hp-qidongzi.png

Figure. The activity of the promoters

User Reviews

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