Difference between revisions of "Part:BBa K2624000:Experience"

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===Applications of BBa_K2624000===
 
===Applications of BBa_K2624000===
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To improve the selectivity of our therapy system, we utilize hTERT, the cancer-specific promoter, and HULC, the HCC-specific promoter, to open an AND-gated system to target HCC (Hepatocellular carcinoma). pGL3-Basic vector, a promoterless vector is used for measuring the activity of promoter with a luciferase assay. We determined the activity of the two promoters by it. Experimental results show that the promoter has a good startup efficiency.
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To improve the selectivity of our system, we utilized hTERT, the cancer-specific promoter, along with HULC, a HCC(Hepatocellular carcinoma)-specific promoter, to open an "AND" gate system to target HCC. We used pGL3-Basic vector, a promoter-less vector for the luciferase assay to determine the transcriptional activity of these promoters. We added the promoters on pGL3-Basic vector and measure the OD value of the luciferase activity. We chose SV40, a highly activated promoter in both cancer cells and normal cells as positive control (PGL3-CON). Results show that hTERT promoter has a good efficiency in cancer cells.
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"https://static.igem.org/mediawiki/2018/5/56/T--CPU_CHINA--hp-qidongzi.png"
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https://static.igem.org/mediawiki/2018/archive/5/56/20181017205901%21T--CPU_CHINA--hp-qidongzi.png
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Figure. The activity of the promoters
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 03:33, 8 December 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2624000

To improve the selectivity of our system, we utilized hTERT, the cancer-specific promoter, along with HULC, a HCC(Hepatocellular carcinoma)-specific promoter, to open an "AND" gate system to target HCC. We used pGL3-Basic vector, a promoter-less vector for the luciferase assay to determine the transcriptional activity of these promoters. We added the promoters on pGL3-Basic vector and measure the OD value of the luciferase activity. We chose SV40, a highly activated promoter in both cancer cells and normal cells as positive control (PGL3-CON). Results show that hTERT promoter has a good efficiency in cancer cells.

20181017205901%21T--CPU_CHINA--hp-qidongzi.png

Figure. The activity of the promoters

User Reviews

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