Difference between revisions of "Part:BBa K2832003:Design"
 
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===Design Notes===
 
===Design Notes===
 
https://static.igem.org/mediawiki/parts/b/b0/PspA_design_diagram.png
 
https://static.igem.org/mediawiki/parts/b/b0/PspA_design_diagram.png
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PpspA Design Diagram. The pspA promoter sequence binds PspF activated via the PspABCDE pathway. The pathway detects membrane damage and loss of proton motive force. mRFP1 signal indicates successful activation of the pathway.
  
 
https://static.igem.org/mediawiki/parts/6/61/PspA_plasmid.png
 
https://static.igem.org/mediawiki/parts/6/61/PspA_plasmid.png
  
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<p>FSU Promoter BBa_K2832003 inside plasmid pSB1C3. The pspA promoter sequence binds PspF activated via the PspABCDE pathway. The pathway detects membrane damage and loss of proton motive force. mRFP1 signal indicates successful activation of the pathway.<p/>
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FSU Promoter BBa_K2832003 inside plasmid pSB1C3.
 
===Source===
 
===Source===
  

Latest revision as of 20:56, 1 December 2018


PpspA - Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PspA_design_diagram.png PpspA Design Diagram. The pspA promoter sequence binds PspF activated via the PspABCDE pathway. The pathway detects membrane damage and loss of proton motive force. mRFP1 signal indicates successful activation of the pathway.

PspA_plasmid.png

FSU Promoter BBa_K2832003 inside plasmid pSB1C3.

Source

E. coli K12 genome.

References