Difference between revisions of "Part:BBa K2832003:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | https://static.igem.org/mediawiki/parts/b/b0/PspA_design_diagram.png | |
| − | + | PpspA Design Diagram. The pspA promoter sequence binds PspF activated via the PspABCDE pathway. The pathway detects membrane damage and loss of proton motive force. mRFP1 signal indicates successful activation of the pathway. | |
| + | https://static.igem.org/mediawiki/parts/6/61/PspA_plasmid.png | ||
| + | FSU Promoter BBa_K2832003 inside plasmid pSB1C3. | ||
===Source=== | ===Source=== | ||
Latest revision as of 20:56, 1 December 2018
PpspA - Inducible Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 115
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 13
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PpspA Design Diagram. The pspA promoter sequence binds PspF activated via the PspABCDE pathway. The pathway detects membrane damage and loss of proton motive force. mRFP1 signal indicates successful activation of the pathway.
FSU Promoter BBa_K2832003 inside plasmid pSB1C3.
Source
E. coli K12 genome.