Difference between revisions of "Part:BBa K2832001:Design"

 
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===Design Notes===
 
===Design Notes===
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Design considerations...
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https://static.igem.org/mediawiki/parts/f/f6/PosmC_Design_Diagram.png
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PosmC Design Diagram. The osmC promoter sequence binds RcsB or NhaR. RcsB pathway is important for cell growth functions. NhaR is activated via increased concentration of soluble ions, possible through the opening of a mechanosensitive channel. mRFP1 signal indicates successful activation of the pathway.
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https://static.igem.org/mediawiki/parts/3/3f/PosmC_Plasmid_Map.png
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FSU Promoter BBa_K2832001 inside plasmid pSB1C3
  
  

Revision as of 20:55, 1 December 2018


PosmC - Inducible Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PosmC_Design_Diagram.png PosmC Design Diagram. The osmC promoter sequence binds RcsB or NhaR. RcsB pathway is important for cell growth functions. NhaR is activated via increased concentration of soluble ions, possible through the opening of a mechanosensitive channel. mRFP1 signal indicates successful activation of the pathway. PosmC_Plasmid_Map.png FSU Promoter BBa_K2832001 inside plasmid pSB1C3


Source

E. coli K12

References