Difference between revisions of "Part:BBa K2598027"

 
 
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<partinfo>BBa_K2598027 short</partinfo>
 
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This is a bacterial flavin-containing monooxygenase(bFMO). It can catalyse tryptophan and render it blue. This part van be used as a reporter gene and, in our project, paint.
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This is a bacterial flavin-containing monooxygenase(bFMO). It can catalyse tryptophan and render it blue. This part van be used as a reporter gene and, in our project, paint. It is part of the GRB system, see more information from [https://parts.igem.org/Part:BBa_J23106 RGB System]
  
 
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<partinfo>BBa_K2598027 parameters</partinfo>
 
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===Characterization===
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<b>Figure 1</b> shows results of gel electrophoresis of various parts after PCR with primer VF2\VR. The distance between primer binding sites and both ends of the parts are approximately 150 bp, thus rendering the product about 300 bp longer. The picture is edited to show a more compact photo.
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<div>[[File:T—UCAS-China—figure6 2018.tif|700px|thumb|center|<b>Figure 1:</b> Results of gel electrophoresis of various parts after PCR with primer VF2\VR]]</div>
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<b>Figure 2</b> shows colors produced by enzymes that can combine with substrate in the medium. We changed the output genes of our light control system to genes encoding three kinds of enzymes, including gusA（BBa_K330002）, lacZ（BBa_I732005） and bFMO（BBa_K2598027） that can combine with substrate in the medium to produce red, green and blue color respectively.
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<div>[[File:T—UCAS-China—enzyme2018.png|700px|thumb|center|<b>Figure 2:</b> Colors produced by enzymes that can combine with substrate in the medium]]</div>

Latest revision as of 03:48, 18 October 2018


bFMO(bacterial flavin-containing monooxygenase)

This is a bacterial flavin-containing monooxygenase(bFMO). It can catalyse tryptophan and render it blue. This part van be used as a reporter gene and, in our project, paint. It is part of the GRB system, see more information from RGB System

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
    Illegal AgeI site found at 180
    Illegal AgeI site found at 484
    Illegal AgeI site found at 814
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Figure 1 shows results of gel electrophoresis of various parts after PCR with primer VF2\VR. The distance between primer binding sites and both ends of the parts are approximately 150 bp, thus rendering the product about 300 bp longer. The picture is edited to show a more compact photo.


Figure 2 shows colors produced by enzymes that can combine with substrate in the medium. We changed the output genes of our light control system to genes encoding three kinds of enzymes, including gusA（BBa_K330002）, lacZ（BBa_I732005） and bFMO（BBa_K2598027） that can combine with substrate in the medium to produce red, green and blue color respectively.

Figure 2: Colors produced by enzymes that can combine with substrate in the medium