Difference between revisions of "Part:BBa K2719008:Design"
 
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===References===
 
===References===
 +
<p>M.N.M Walter. et al (2010).<i> Mesenchymal stem cell-conditioned medium accelerates skin wound healing: An in vitro study of fibroblast and keratinocyte scratch assays</i>. Doi:10:1016 /j.excr.2010.02.26</p>
 +
<p>Ahsan SM. et al (2017).<i> Chitosan as biomaterial in drug delivery and tissue engineering </i>doi: 10.1016/j.ijbiomac.2017.08.140.</p>
 +
<p>Gainsford T. et al (1998). <i>Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells.</i> 10;93(25):14564-8.</p>
 +
<p>[1] Jian Feng Li, Jie Zhang, Zhen Zhang, Yun Long Hu and Shuang Quan Zhang. (2010). <i>Efficient Expression of Bioactive Human Leptin in Escherichia coli in Soluble Fusion Form</i>. March 29, 2018, of NCBI Website: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001832/</p>
 +
<p>[1.1] <i>Leptin sequence [NC_000007.14] </i>https://www.ncbi.nlm.nih.gov/nuccore/NC_000007.14?report=genbank&from=128241201&to=128257629</p>
 +
<p>[2] Paul Duchey. (2011).  <i>Comprehensive Biomaterials</i>. 23 May, 2018, de ELSEVIER Sitio web: https://books.google.com.mx/books?id=oa8YpRsD1kkC&pg=RA1-PA313&lpg=RA1-PA313&dq=heparin+scaffold+leptin&source=bl&ots=ldZc_piKlc&sig=te2DfWxtO633nFX2bqC2pvCr8d0&hl=es&sa=X&ved=0ahUKEwicpufNxo3bAhUEaq0KHVyqCEQQ6AEIRjAD#v=onepage&q=heparin%20scaffold%20leptin&f=false</p>
 +
 +
<p>[3] Poeggeler Burkhard, Carla Schulz, et. al. (2009). <i>Leptin and skin: A new frontier</i>. 23, May, 2018, de ResearchGate Sitio web: https://www.researchgate.net/publication/26670611_Leptin_and_the_skin_A_new_frontier</p>
 +
<p>[4] Spadaccio C., Rainer A., et. al. (2010). <i>Heparin-releasing scaffold for stem cells: a differentiating device for vascular aims</i>. 23 May, 2018, de NCBI Sitio web:
 +
https://www.ncbi.nlm.nih.gov/pubmed/20632865</p>
 +
<p>[9] Frank, S., Kolob, N., Pfeilschifter, J.. (2000). <i>Leptin enhances wound re-epithelialization and constitutes a direct function of leptin in skin repair</i>. June 6th, 2018, de JCI Sitio web: https://www.jci.org/articles/view/9148</p>
 +
<p>[10] Tourkantonis, I, et. al. (2013). <i>The Role of Leptin in Cancer Pathogenesis</i>. Web site: https://pdfs.semanticscholar.org/cb9c/b576b1565ac55ff6e31c4c76478898504904.pdf</p>
 +
<p>[11] https://www.fda.gov/downloads/Drugs/DrugSafety/PostmarketDrugSafetyInformationforPatientsandProviders/UCM142821.pdf</p>
 +
 +
<p>[12] http://www.ema.europa.eu/docs/es_ES/document_library/EPAR_-_Product_Information/human/000212/WC500050144.pdf</p>
 +
 +
<p>[13] Tsai Minglun, Akihiro Asakawa, et. al. (2012). 08 June, 2018, of NCBI Web site: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3602982/</p>

Latest revision as of 03:41, 18 October 2018


Leptin

This is originated from Hommo sapiens, the original sequence is from this species but was optimized for E.coli BL21 (DE3) and to be cloned by RFC10 on pSB1C3. It mainly consists on a-helix. An histidine tag (6xHis) was added to the protein to facilitate its purification.

"T--TecCEM--Lep3DStructure.png"

Figure 7. Leptin 3D prediction modelled with Swiss Model


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for E.coli.


Source

NCBI Genomic Library

References

M.N.M Walter. et al (2010). Mesenchymal stem cell-conditioned medium accelerates skin wound healing: An in vitro study of fibroblast and keratinocyte scratch assays. Doi:10:1016 /j.excr.2010.02.26

Ahsan SM. et al (2017). Chitosan as biomaterial in drug delivery and tissue engineering doi: 10.1016/j.ijbiomac.2017.08.140.

Gainsford T. et al (1998). Leptin can induce proliferation, differentiation, and functional activation of hemopoietic cells. 10;93(25):14564-8.

[1] Jian Feng Li, Jie Zhang, Zhen Zhang, Yun Long Hu and Shuang Quan Zhang. (2010). Efficient Expression of Bioactive Human Leptin in Escherichia coli in Soluble Fusion Form. March 29, 2018, of NCBI Website: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001832/

[1.1] Leptin sequence [NC_000007.14] https://www.ncbi.nlm.nih.gov/nuccore/NC_000007.14?report=genbank&from=128241201&to=128257629

[2] Paul Duchey. (2011). Comprehensive Biomaterials. 23 May, 2018, de ELSEVIER Sitio web: https://books.google.com.mx/books?id=oa8YpRsD1kkC&pg=RA1-PA313&lpg=RA1-PA313&dq=heparin+scaffold+leptin&source=bl&ots=ldZc_piKlc&sig=te2DfWxtO633nFX2bqC2pvCr8d0&hl=es&sa=X&ved=0ahUKEwicpufNxo3bAhUEaq0KHVyqCEQQ6AEIRjAD#v=onepage&q=heparin%20scaffold%20leptin&f=false

[3] Poeggeler Burkhard, Carla Schulz, et. al. (2009). Leptin and skin: A new frontier. 23, May, 2018, de ResearchGate Sitio web: https://www.researchgate.net/publication/26670611_Leptin_and_the_skin_A_new_frontier

[4] Spadaccio C., Rainer A., et. al. (2010). Heparin-releasing scaffold for stem cells: a differentiating device for vascular aims. 23 May, 2018, de NCBI Sitio web: https://www.ncbi.nlm.nih.gov/pubmed/20632865

[9] Frank, S., Kolob, N., Pfeilschifter, J.. (2000). Leptin enhances wound re-epithelialization and constitutes a direct function of leptin in skin repair. June 6th, 2018, de JCI Sitio web: https://www.jci.org/articles/view/9148

[10] Tourkantonis, I, et. al. (2013). The Role of Leptin in Cancer Pathogenesis. Web site: https://pdfs.semanticscholar.org/cb9c/b576b1565ac55ff6e31c4c76478898504904.pdf

[11] https://www.fda.gov/downloads/Drugs/DrugSafety/PostmarketDrugSafetyInformationforPatientsandProviders/UCM142821.pdf

[12] http://www.ema.europa.eu/docs/es_ES/document_library/EPAR_-_Product_Information/human/000212/WC500050144.pdf

[13] Tsai Minglun, Akihiro Asakawa, et. al. (2012). 08 June, 2018, of NCBI Web site: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3602982/