Difference between revisions of "Part:BBa K2787012"
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===Usage and Biology===
 
===Usage and Biology===
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A plasmid with GFP under control of the A2 orthogonal RBS with the TJU orthogonal ribosome on it is constructed.
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[[File:Shanghaitech2018-oribo-orbs-07-07.png|700px|center]]
 
[[File:ShanghaiTech2018-A2_rbs.png|700px|center]]
 
[[File:ShanghaiTech2018-A2_rbs.png|700px|center]]
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The GFP under control of A2 orthogonal RBS is tested within different 16s rRNAs. The fluorescence with A2 orthogonal ribosome is the best among host ribosomes and A2,B8,C9 ribosomes, which shows the good orthogonality of A2 16s rRNA with A2 orthogonal RBS.
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[[File:A2-B8-C9-Orthogonality.png|700px|center]]
 
[[File:A2-B8-C9-Orthogonality.png|700px|center]]
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The fluorescence under the test with 3 different orthogonal RBS and 3 different orthogonal ribosmes. It shows that the orthogonality of A2 ribosome and A2 RBS is one of the best ones.
 
[[File:ShanghaiTech-TJU 16S.png|700px|center]]
 
[[File:ShanghaiTech-TJU 16S.png|700px|center]]
 +
The GFP under control of different orthogonal RBS is tested within TJU 16s rRNAs. There is not huge differences between the fluorescence with different orthogonals. It shows that the B8 orthogonal RBS is orthogonal to A2,TJU and C9 16s ribosomes.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 03:37, 18 October 2018


GFP under A2 orthogonal RBS with orthogonal 16S rRNA BBa_K2787040

The 16S rRNA BBa_K2787040 under constitutive promoter BBa_J23100(BBa_K2787008) and pCons-oRBSA2-GFP(BBa_K2787000) are linked into one plasmid to quantify the orthogonality of the TJU and A2 ribosome system.


Usage and Biology

A plasmid with GFP under control of the A2 orthogonal RBS with the TJU orthogonal ribosome on it is constructed.

Shanghaitech2018-oribo-orbs-07-07.png
ShanghaiTech2018-A2 rbs.png

The GFP under control of A2 orthogonal RBS is tested within different 16s rRNAs. The fluorescence with A2 orthogonal ribosome is the best among host ribosomes and A2,B8,C9 ribosomes, which shows the good orthogonality of A2 16s rRNA with A2 orthogonal RBS.


A2-B8-C9-Orthogonality.png

The fluorescence under the test with 3 different orthogonal RBS and 3 different orthogonal ribosmes. It shows that the orthogonality of A2 ribosome and A2 RBS is one of the best ones.

ShanghaiTech-TJU 16S.png

The GFP under control of different orthogonal RBS is tested within TJU 16s rRNAs. There is not huge differences between the fluorescence with different orthogonals. It shows that the B8 orthogonal RBS is orthogonal to A2,TJU and C9 16s ribosomes. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 797
    Illegal NheI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1660
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1674
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706