Difference between revisions of "Part:BBa K2729012"

 
 
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<partinfo>BBa_K2729012 short</partinfo>
 
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==Usage and Biology==
 
Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
 
Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
  
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==Characterization==
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===Usage and Biology===
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The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.
  
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[[File:Tokyotech 2018 EP (E,prME).png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis''' ]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2729012 SequenceAndFeatures</partinfo>
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As shown in Figure 1, the band of DENV3 prME come around 2,000 bp.
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===Analysis===
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Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for the transfection.
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If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.
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==Sequence and Features==
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<partinfo>BBa_K2729012 SequenceAndFeatures</partinfo>
  
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==Reference==
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===Functional Parameters===
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- [https://pubs.acs.org/doi/10.1021/bi500724k Membranotropic regions of the dengue virus prM protein]
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<partinfo>BBa_K2729012 parameters</partinfo>
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Latest revision as of 03:35, 18 October 2018


DENV3 ancC-prM-E (Dengue Virus Serotype III)

Usage and Biology

Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.

Figure 1: Result of the Electrophoresis


As shown in Figure 1, the band of DENV3 prME come around 2,000 bp.

Analysis

Our team successfully validated that the sequence was synthesized, and obtained enough amount of it for the transfection.

If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

- Membranotropic regions of the dengue virus prM protein