Difference between revisions of "Part:BBa K2729004"

Latest revision as of 03:32, 18 October 2018

DENV3 E (Envelope Protein | Dengue Virus Serotype III)

Usage and Biology

Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)

As shown in Figure 1, the band of DENV3 E come around 1,500 bp. The actual length is 1479 bp.

Analysis

Our team successfully validated that the sequence of DENV3 E was synthesized.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1307
Illegal XhoI site found at 868
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 250
Illegal SapI.rc site found at 1078

Reference

- NCBI Gene Database

@@ Line 10: / Line 10: @@
 The sequence is derived from native dengue virus and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
-[[File:Tokyotech 2018 EP (E,prME).png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis''' ]]
+[[File:Tokyotech 2018 EP (E,prME).png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)''' ]]
 <br>
-As shown in Figure 1, the band of DENV3 E come around 1,500 bp.
+As shown in Figure 1, the band of DENV3 E come around 1,500 bp. The actual length is 1479 bp.
 <br style="clear: both" />