Difference between revisions of "Part:BBa K2729004"
 
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<partinfo>BBa_K2729004 short</partinfo>
 
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===Usage and Biology===
 
Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
 
Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
  
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===Characterization===
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===Usage and Biology===
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The sequence is derived from native dengue virus and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2729004 SequenceAndFeatures</partinfo>
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[[File:Tokyotech 2018 EP (E,prME).png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)''' ]]
  
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===Functional Parameters===
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As shown in Figure 1, the band of DENV3 E come around 1,500 bp. The actual length is 1479 bp.
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<partinfo>BBa_K2729004 parameters</partinfo>
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==Chactarization==
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===Analysis===
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The DENV E protein is a class II fusion protein, essential for attachment, membrane fusion, and assembly.A series of conformational changes occurring in the DENV E protein driven by the endosomal low-pH gives place to the fusion of the viral and endosomal membranes.
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Our team successfully validated that the sequence of DENV3 E was synthesized.
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===Sequence and Features===
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<partinfo>BBa_K2729004 SequenceAndFeatures</partinfo>
  
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==Reference==
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===Reference===
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- [https://www.sciencedirect.com/science/article/pii/S0005273611001970 Nemésio H, Palomares-Jerez F, Villalaín J. The membrane-active regions of the dengue virus proteins C and E. Biochim Biophys Acta. 2011 Oct;1808(10):2390-402.doi: 10.1016/j.bbamem.2011.06.019. Epub 2011 Jul 13. PubMed PMID: 21777566.]
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- [https://www.ncbi.nlm.nih.gov/gene/507572 NCBI Gene Database]

Latest revision as of 03:32, 18 October 2018


DENV3 E (Envelope Protein | Dengue Virus Serotype III)

Usage and Biology

Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)


As shown in Figure 1, the band of DENV3 E come around 1,500 bp. The actual length is 1479 bp.

Analysis

Our team successfully validated that the sequence of DENV3 E was synthesized.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1307
    Illegal XhoI site found at 868
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 250
    Illegal SapI.rc site found at 1078

Reference

- NCBI Gene Database