Difference between revisions of "Part:BBa K2729001"

Latest revision as of 03:32, 18 October 2018

DENV2 C (Capsid Protein | Dengue Virus Serotype Ⅱ)

Usage and Biology

Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.

Characterization

The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)

As shown in Figure 1, the band of DENV2 C come around 300 bp. It corresponds to its actual length.

Analysis

Our team successfully validated that the sequence of DENV2 C was synthesized, and obtained enough amount of it for transfection.

If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

- NCBI Gene Database

- Properties and Functions of the Dengue Virus Capsid Protein.

@@ Line 12: / Line 12: @@
 The sequence is derived from native dengue virus and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
-[[File:TokyoTech 2018 EPresult.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis''' ]]
+[[File:Tokyotech electrophoresis.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)'''  ]]
 <br>
-As shown in Figure 1, the band of DENV2 C come around 300 bp.
+As shown in Figure 1, the band of DENV2 C come around 300 bp. It corresponds to its actual length.
 <br style="clear: both" />
@@ Line 21: / Line 21: @@
 Our team successfully validated that the sequence of DENV2 C was synthesized, and obtained enough amount of it for transfection.
+If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.
 ===Sequence and Features===