Difference between revisions of "Part:BBa K2729001"
 
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===Usage and Biology===
 
===Usage and Biology===
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Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
  
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Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
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Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.
  
 
===Characterization===
 
===Characterization===
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Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.
 
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実験の内容
 
  
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[[File:T--Tokyo_Tech--test-electrop.gif|thumb|left|300px| '''Figure 1:''' '''Result of the qualitative experiment''' - description comes here. ]]
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The sequence is derived from native dengue virus and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
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[[File:Tokyotech electrophoresis.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)''' ]]
  
 
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<br>
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As shown in Fig. 1, ~.  
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As shown in Figure 1, the band of DENV2 C come around 300 bp. It corresponds to its actual length.
 
<br style="clear: both" />
 
<br style="clear: both" />
  
 
===Analysis===
 
===Analysis===
  
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分析結果
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Our team successfully validated that the sequence of DENV2 C was synthesized, and obtained enough amount of it for transfection.
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If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.
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===Sequence and Features===
 
===Sequence and Features===
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===Reference===
 
===Reference===
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参考文献
 
 
- [https://www.ncbi.nlm.nih.gov/gene/507572 NCBI Gene Database]
 
- [https://www.ncbi.nlm.nih.gov/gene/507572 NCBI Gene Database]
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- [https://www.ncbi.nlm.nih.gov/pubmed/?term=Properties+and+Functions+of+the+Dengue+Virus+Capsid+Protein Byk LA, GamarnikAV. Properties and Functions of the Dengue Virus Capsid Protein. Annu Rev Virol. 2016 Sep 29;3(1):263-281. Epub 2016 Aug 3. Review.PubMed PMID: 27501261; PubMed Central PMCID: PMC5417333.]
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- [https://www.ncbi.nlm.nih.gov/pubmed/?term=Properties+and+Functions+of+the+Dengue+Virus+Capsid+Protein Properties and Functions of the Dengue Virus Capsid Protein.]
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Latest revision as of 03:32, 18 October 2018


DENV2 C (Capsid Protein | Dengue Virus Serotype Ⅱ)

Usage and Biology

Flavivirus capsid (C) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Mature DENV capsid is a highly basic protein of 12 kDa that forms homodimers in solution with affinity for both nucleic acids and lipid membranes. The 100-residue monomer contains 26 basic amino acids and only 3 acidic residues.

Characterization

The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)


As shown in Figure 1, the band of DENV2 C come around 300 bp. It corresponds to its actual length.

Analysis

Our team successfully validated that the sequence of DENV2 C was synthesized, and obtained enough amount of it for transfection.

If it works well in the process of pseudo-virus production and also infection into host cells as expected, the fluorescence intensity can be observed robustly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

- NCBI Gene Database

- Properties and Functions of the Dengue Virus Capsid Protein.