Difference between revisions of "Part:BBa K2729005"

 
 
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<partinfo>BBa_K2729005 short</partinfo>
 
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===Usage and Biology===
 
Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
 
Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
  
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===Characterization===
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===Usage and Biology===
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The sequence is derived from native dengue virus and optimized for <i>Homo Sapiens</i> using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2729005 SequenceAndFeatures</partinfo>
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[[File:Tokyotech 2018 EP (E,prME).png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis (Marker: lambda HindIII)''' ]]
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As shown in Figure 1, the band of DENV4 E come around 1,500 bp. The actual length is 1485 bp.
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===Analysis===
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Our team successfully validated that the sequence of DENV4 E was synthesized.
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===Sequence and Features===
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<partinfo>BBa_K2729005 SequenceAndFeatures</partinfo>
  
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===Reference===
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===Functional Parameters===
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- [https://www.ncbi.nlm.nih.gov/gene/507572 NCBI Gene Database]
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<partinfo>BBa_K2729005 parameters</partinfo>
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Latest revision as of 03:29, 18 October 2018


DENV4 E (Envelope Protein | Dengue Virus Serotype IV)

Usage and Biology

Flavivirus Envelope (E) is a structural protein responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The sequence is derived from native dengue virus and optimized for Homo Sapiens using IDT Codon Optimization Tool. Our team checked the sequence length of synthesized oligo DNA and characterized it.

Figure 1: Result of the Electrophoresis (Marker: lambda HindIII)


As shown in Figure 1, the band of DENV4 E come around 1,500 bp. The actual length is 1485 bp.

Analysis

Our team successfully validated that the sequence of DENV4 E was synthesized.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1249

Reference

- NCBI Gene Database