Difference between revisions of "Part:BBa K2700001"
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<partinfo>BBa_K2700001 short</partinfo> | <partinfo>BBa_K2700001 short</partinfo> | ||
− | + | This is our pt181-antisense-RNA transcript that will block both transcription and translation at the pt181 promoter (Part:BBa_K2700002) site. Once pt181-antisense is expressed, it will bind to the promoter region and cause a physical fold to occur. This fold is a stem-like structure that will block the RBS as well as act as a terminator to prevent further transcription to occur. | |
+ | |||
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Revision as of 03:24, 18 October 2018
Antisense-RNA for transcriptional/translational repression
This is our pt181-antisense-RNA transcript that will block both transcription and translation at the pt181 promoter (Part:BBa_K2700002) site. Once pt181-antisense is expressed, it will bind to the promoter region and cause a physical fold to occur. This fold is a stem-like structure that will block the RBS as well as act as a terminator to prevent further transcription to occur.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]