Difference between revisions of "Part:BBa K2859000:Experience"

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We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.We confirmed that the ligation was successfully done by running gel with minicprepped DNA cut with EcoR1 (about 2.5kb). The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.
  
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Afther synthesizing the part flanked by biobrick prefix and suffix, we used digested the part and pSB1C3 vector with EcoR1 and Pst1 and ligated the part into the pSB1C3 vector. The resulting plasmid was transformed into E.Coli DH5-α. They were grown overnight at 37°C. The samples were prepared for SDS page gel. The expression of Penetratin-Insulin-Flag tag complex was evaluated with SDS page gel.
 
  
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https://static.igem.org/mediawiki/2018/8/81/T--HAFS--partgel3.png
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Figure1. 1.2% Agarose Gel electrophoresis result of pSB1C3 cut with EcoR1
  
 
https://res.cloudinary.com/dt6jjveot/image/upload/SDS.jpg
 
https://res.cloudinary.com/dt6jjveot/image/upload/SDS.jpg
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Figure2. 10% SDS page gel result showing the expression of protein

Latest revision as of 03:23, 18 October 2018


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UNIQ28f54544979bc8f4-partinfo-00000000-QINU UNIQ28f54544979bc8f4-partinfo-00000001-QINU

We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.We confirmed that the ligation was successfully done by running gel with minicprepped DNA cut with EcoR1 (about 2.5kb). The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.


T--HAFS--partgel3.png


Figure1. 1.2% Agarose Gel electrophoresis result of pSB1C3 cut with EcoR1

SDS.jpg

Figure2. 10% SDS page gel result showing the expression of protein