Difference between revisions of "Part:BBa K2859000:Experience"

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We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.We confirmed that the ligation was successfully done by running gel with minicprepped DNA cut with EcoR1 (about 2.5kb). The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.
  
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https://static.igem.org/mediawiki/2018/8/81/T--HAFS--partgel3.png
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Figure1. 1.2% Agarose Gel electrophoresis result of pSB1C3 cut with EcoR1
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https://res.cloudinary.com/dt6jjveot/image/upload/SDS.jpg
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Figure2. 10% SDS page gel result showing the expression of protein

Latest revision as of 03:23, 18 October 2018


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User Reviews

UNIQac5c3252f0d2d07b-partinfo-00000000-QINU UNIQac5c3252f0d2d07b-partinfo-00000001-QINU

We synthesised the part flanked by bioBrick prefix and suffix. Then, the synthesized gblock was ligated into pSB1C3 by restriction digest with EcoR1 and Pst1 and transformed into E.Coli DH5-α.We confirmed that the ligation was successfully done by running gel with minicprepped DNA cut with EcoR1 (about 2.5kb). The transformed E.coli was grown at 37°C. The expression of Penetratin-Insulin-Flag tag complex was evaluated by SDS page gel.


T--HAFS--partgel3.png


Figure1. 1.2% Agarose Gel electrophoresis result of pSB1C3 cut with EcoR1

SDS.jpg

Figure2. 10% SDS page gel result showing the expression of protein